The parental DNA template is eliminated by treating with DpnI, which destroys the methylated template DNA5. The resulting nicked DNA is transformed into proficient E. coli cells for nick repair service. The extracts inhibited the mutagenicity induced by both direct- and oblique-acting mutagens, nevertheless the inhibition was larger for S9-dependent https://mutagenesis08642.wikibyby.com/307894/indicators_on_site_directed_mutagenesis_you_should_know