Very hot-begin/cold-finish PCR is achieved with new hybrid polymerases that are inactive at ambient temperature and are immediately activated at elongation temperature. Touchdown PCR (Step-down PCR): a variant of PCR that aims to reduce nonspecific history by gradually lowering the annealing temperature as PCR biking progresses. The annealing temperature https://vigilantcbd.ageeksblog.com/5819896/pcr-pure-no-further-a-mystery